Figure 3
From: Bioluminescent imaging of ABCG2 efflux activity at the blood-placenta barrier
ABCG2 inhibition increases D-luciferin accumulation and bioluminescent signal in choriocarcinoma cells.
(A) D-luciferin (2 mM) accumulation in BeWo cell line, with and without ABCG2 inhibitors Ko143 (100 nM, 5 μM) and fumitrogen C (FTC; 100 nM, 5 μM) measured by flow cytometry. (B) D-luciferin (2 mM) accumulation in Jeg-3. (C) D-luciferin (2 mM) accumulation in H460-MX20. (D) To measure the effect of ABCG2 on bioluminescence, all three cell lines were transiently transduced with a baculovirus (Bacmam) containing firefly luciferase. Bioluminescence with and without 5 μM Ko143 is reported. All values are normalized to the 5 μM Ko143 condition. Data represent means ± SD of three experiments (*p < 0.05 **p < 0.01, ***p < 0.001, ****p < 0.0001 by Student’s t test).
