Figure 4

In vivo bioluminescent pregnant mouse model.

(A) Illustration of the breeding strategy to generate pregnant mice carrying placentae and fetuses expressing fLuc. (B,C) Diagrammatic representation of the BPB barrier with the hypothesized distribution of d-luciferin (B) before and (C) after ABCG2 inhibition. (D) Bioluminescence images (left to right) of a male CAG-fLuc mouse, non-pregnant female FVB mouse, pregnant FVB mouse and pregnant FVB mouse imaged immediately post euthanasia with peritoneum opened to visualize uterus, all after being administered 5 mg/kg D-luciferin i.p. for 20 min. (E) Bioluminescence images from (left to right) of intact uterus demonstrating BLI signal from fetuses and placentae and female mouse after uterus was removed with no signal seen, after 5 mg/kg IP d-luciferin for 20 minutes.