Figure 1
From: Linking suckling biomechanics to the development of the palate

Postnatal mechanical environment predicts the emergence of cartilage in the midpalatal suture.
Aniline blue staining of representative tissue sections from (A) E18 and (F) P1 mouse palate. Meshed three-dimensional model of (B) E18 and (G) P1 mouse palate. The extents of deflection (C,H), distortional strain (D,I) and hydrostatic strain (E,J) were mapped. (K,M) Safranin O/Fast green staining and (L,N) Sox9 immunostaining on representative sections through P1 and P3 mouse palate. Abbreviations: pp = palatal process; b = bone; ct = connective tissue; nc = nasal cavity; oc = oral cavity. Scale bar = 50 μm. Note: all images are oriented with the nasal surface of the palatal bone facing upwards and the oral surface facing downwards.