Figure 4

Cre recombination activity detected in the choroid plexus but not in the hypothalamus of Ins2-Cre mice.

(a) X-gal staining of coronal brain sections showing Cre recombination activity in the choroid plexus of Ins2-Cre;Rosa-LacZ mice. The blue signals in the choroid plexus in the lateral (LV) and third ventricles (V3) are indicated by black arrow heads. No significant staining was detected in the hypothalamus (HYT). Scale bar, 250 μm. (b) Representative coronal brain sections of Ins2-Cre;Rosa-tdTomato mice showing that tdTomato was expressed in the choroid plexus in the LV and V3 and undetectable in the anterior, tuberal and posterior hypothalamus. ARH, arcuate hypothalamic nucleus; DMH, dorsomedial nucleus of the hypothalamus; HPF, hippocampal formation; PVH, paraventricular hypothalamic nucleus; SCH, suprachiasmatic nucleus; LHA, lateral hypothalamic area; MBO, mammillary body; ME, median eminence; Opt, optic tract; PH, posterior hypothalamic nucleus; TH, thalamus. Scale bar, 200 μm.