Figure 5

Bif-1 regulates lipid turnover in adipose tissue and liver.

(a) Plasma levels of free fatty acid (FFA) from mice fed with the indicated diet for 23 weeks in resting and 16 h-fasted states were measured (n = 4). (b) Lysates prepared from white adipose tissue collected from the same animals in (a) were subjected to immunoblotting with the indicated antibodies. Same lysates were also used in Fig. 6b. (c) Plasma levels of glycerol from 6-week old mice fed with normal CD were measured using glycerol reagent (Sigma). Statistical significance was determined using Student’s t-test. (d) Lysates prepared from white adipose tissue collected from the same animals in (c) were subjected to immunoblotting with the indicated antibodies. Same lysates were also used in Fig. 4a. (e,f) Representative images (e) and quantification (f) of ORO stain of the liver sections from mice fed with CD for 20 weeks, fasted overnight or fasted overnight and then refed for 24 h. Scale bars represent 100 μm.