Figure 3 | Scientific Reports

Figure 3

From: MicroRNA-130b improves renal tubulointerstitial fibrosis via repression of Snail-induced epithelial-mesenchymal transition in diabetic nephropathy

Figure 3

MiR-130b ablation enhances Snail-induced EMT in vitro.

(a) MiR-130b inhibitor increased the expression of SNAIL and co-localized with E-CADHERIN (double arrows) (immunofluorescence). (b) Decreased E-CADHERIN by quantification of the staining intensity. (c) MiR-130b inhibitor upregulated the mRNA level of Snail, Vimentin and Collagen IV, but downregulated E-cadherin by qRT-PCR; miR-130b inhibitor increased SNAIL, VIMENTIN and COLLAGEN IV but decreased E-CADHERIN by (d) Western blot analysis. (e) MiR-130b inhibitor induced phenotypic changes of NRK-52E cells with elongated spindle-shaped cell bodies like fibroblasts by SEM. (f) Increased migrated cells treated with miR-130b inhibitor by transwell assay. (g) Longer invaded distances in NRK-52E cells treated with miR-130b inhibitor by wound healing assay. Results are presented as mean ± SD of three independent experiments. **P < 0.01; #P < 0.0001. miR-iNC: miRNA inhibitor negative control; miR-130bi: miR-130b inhibitor; SEM, Scanning Electron Microscope.

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