Figure 4 | Scientific Reports

Figure 4

From: MicroRNA-130b improves renal tubulointerstitial fibrosis via repression of Snail-induced epithelial-mesenchymal transition in diabetic nephropathy

Figure 4

MiR-130b mimic suppresses Snail-induced EMT in vitro.

(a) MiR-130b mimic decreased the expression of SNAIL but increased E-CADHERIN (immunofluorescence). (b) Decreased SNAIL but increased E-CADHERIN expression by quantification of the staining intensity. (c) MiR-130b mimic downregulated the mRNA level of Snail, Vimentin and Collagen IV, but upregulated E-cadherin by qRT-PCR. (d) MiR-130b mimic decreased SNAIL, VIMENTIN and COLLAGEN IV but increased E-CADHERIN by Western blot analysis. (e) NRK-52E cells retained the epithelial feature with miR-130b mimic treatment by SEM. (f) Decreased migrated cells with miR-130b mimic treatment by transwell assay. (g) Shorter invaded distance in NRK-52E cells treated with miR-130b mimic by wound healing assay. Results are presented as mean ± SD of three independent experiments. **P < 0.01; #P < 0.0001. miR-NC, miRNA mimic negative control; miR-130bm: miR-130b mimic.

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