Figure 5 | Scientific Reports

Figure 5

From: MicroRNA-130b improves renal tubulointerstitial fibrosis via repression of Snail-induced epithelial-mesenchymal transition in diabetic nephropathy

Figure 5

High glucose inhibits miR-130b and regulates Snail-induced downstream gene expressions in vitro.

(a) High glucose (30 mM) reduced miR-130b expression in a time-dependent manner. (b) High glucose (30 mM) increased the level of Snail, Vimentin and Collagen IV, but decreased E-cadherin by qRT-PCR and (c) Western blot analyses. (d) Increasing concentrations of glucose from 5 to 30 mM reduced miR-130b expression in a dose-dependent manner at 72 h. (e) Increased Snail, Vimentin and Collagen IV, but decreased E-cadherin expression stimulated with increasing concentrations of glucose at 72 h by qRT-PCR and (f) Western blot analyses. Results are presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; #P < 0.0001. HG, high glucose.

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