Figure 7 | Scientific Reports

Figure 7

From: MicroRNA-130b improves renal tubulointerstitial fibrosis via repression of Snail-induced epithelial-mesenchymal transition in diabetic nephropathy

Figure 7

MiR-130b inhibitor increases EMT markers and promotes renal tubulointerstitial fibrosis in vivo.

(a) Decreased miR-130b in plasma and (b) kidney samples of diabetic rats and miR-130b inhibitor further reduced its expression. (c) MiR-130b inhibitor increased Snail, Vimentin, Collagen IV and α-SMA, but decreased E-cadherin by qRT-PCR and (d) Western blot analysis. (e) MiR-130b inhibitor upregulated SNAIL, VIMENTIN, COLLAGEN IV and α-SMA, but decreased E-CADHERIN by immunohistochemistry. (f) Increased renal tubulointerstitial fibrosis in diabetic rats treated with miR-130b inhibitor by MTS. (g) Quantification for immunohistochemistry and (h) interstitial injury score. Results are presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; #P < 0.0001. NC, normal control; DM, diabete mellitus; DM_miR-iNC, diabetic rats treated with miRNA inhibitor negative control; DM_miR-130bi, diabetic rats treated with miR-130b inhibitor; MTS, Masson’ s trichrome stain.

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