Figure 4
From: Cross Reactive Material 197 glycoconjugate vaccines contain privileged conjugation sites

Overlay of all LC-MALDI-MS spectra acquired during the LC run of unconjugated CRM197.
For each identified peak charge, peptide position and average mass are indicated. The major peaks are all corresponding to the singly charged CNBr peptides without any missed cleavages. All of the eight expected peptides were detected. Doubly charged species were also found for larger peptides as well as low abundant signals corresponding to peptides with missed cleavage sites. The survey view illustrates retention time differences of the various peptides.