Figure 6
From: Cross Reactive Material 197 glycoconjugate vaccines contain privileged conjugation sites
Relative quantitation of the seven lysine containing CNBr peptides derived from two ST3 batches.
Each peptide section was quantified individually. The sum of the area under the curve from all detected signals obtained for a given peptide (with and without modifications) was set to 100 %. The percentages presented in the graph are rounded to the full digit. Differences in glycan and free linker loading could be directly compared for each peptide. The results clearly demonstrated that glycan loading was more effective in batch B over the entire protein sequence. Inversely batch A exhibited higher loading of free linker molecules.
