Figure 7
From: Cross Reactive Material 197 glycoconjugate vaccines contain privileged conjugation sites
Exemplary MALDI-ISD spectra of CRM197 peptide 231–314 acquired from the unconjugated control (top) and an ST3 conjugated batch (bottom) after separation by C8 chromatography.
An m/z + 848 shift of the c12-ion observed between the top and bottom spectra indicated K242 to be the major modified lysine residue in this peptide. The ISD-spectra also provided extensive amino acid sequence data of the peptide.
