Figure 8
From: Cross Reactive Material 197 glycoconjugate vaccines contain privileged conjugation sites
(Left): Heatmap showing the lysine residues detected to be conjugated (red label) after proteolytic digestion with trypsin, Glu-C or combinations of trypsin/Glu-C or Glu-C/Asp-N. Grey labelled lysine residues were not observed as being conjugated. Data obtained for four independent conjugates carrying a variety of glycoepitopes (LPG, ST3, PS1 and GLC as represented in each column) showed that several lysine residues were commonly found conjugated. (Right) 3D crystal structure of CRM197 dimer (PDB entry: 4AE0). Lysine residues are labelled blue. Lysine residues that were frequently found conjugated with a glycan in all the samples are labelled in red.
