Figure 3

miR-101 induces apoptosis and cell cycle arrest.
(A) Apoptosis assay in both MCF-7 and MDA-MB-231 cells with or without miR-101 over-expression by Annexin V and PI double staining followed with flow cytometry analysis. (B) DAPI staining to detect apoptotic cells. (C) Cell cycle analysis in both MCF-7 and MDA-MB-231 cells with or without miR-101 over-expression by PI staining followed by flow cytometry analysis. Data were analyzed using Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.