Figure 8 | Scientific Reports

Figure 8

From: MicroRNA-101 targets von Hippel-Lindau tumor suppressor (VHL) to induce HIF1α mediated apoptosis and cell cycle arrest in normoxia condition

Figure 8

HIF1α is attributed to miR-101 mediated apoptosis and cell cycle arrest.

(A) HIF1a level change in MCF-7 cells infected with HIF1a expression vector by western blot. (B) VEGFA level change in MCF-7-miR-101 cells after 48 h 2-MeOE2 treatment by qPCR. (C) Apoptosis assay in MCF-7 cells with HIF1a over-expression by Annexin V and PI double staining followed with flow cytometry analysis. (D) Apoptosis assay in MCF-7-miR-101 cells after treated with 2-MeOE2. (E–G) Percentage of G1, S and G2/M phages (E), G1 phase (F) and S phase (G) in MCF-7-miR-101 cells after 2-MeOE2 treatment. Data were analyzed using Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.

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