Figure 6
HER2/Snail double positive gastric cancer patients have unfavorable outcome.
(A) Representative immunohistochemical staining of HER2 and Snail by respective antibody in human gastric tissues. Original magnification, 100 × . (B) Representative FISH staining of HER2 by probe in human gastric tissues. Original magnification, 400 × . HER2 amplification was determined as a ratio of HER2 (red dots) and chromosome 17 centromere (green dots) signal counts. Ratios < 2 were determined as no amplification with FISH and those >2 as positive amplification with FISH. “a” represents no amplification; “b” represents positive amplification. (C) Chi-square analysis of the correlation of HER2 expression or amplification with Snail expression. (D) Kaplan-Meier Curve analysis of HER2 amplification with OS for patients with gastric cancer by the log-rank test. (E) Kaplan-Meier estimation of Snail overexpression with OS for patients with gastric cancer by the log-rank test. (F) Kaplan-Meier estimation of HER2/Snail double positive expression versus single positive or both negative counterparts with OS for patients with gastric cancer by the log-rank test.
