Figure 2
The extent of DNA compaction by large TRF2 complexes correlates with the volume of the complexes.
(a) Histograms of the length of DNA alone (insert, blue bars, N = 69, 1721.0 ± 61.9 nm), DNA with small (black bars, N = 134, 1704.0 ± 59.0 nm) and large (red bars, N = 51, 1532.0 ± 241.0 nm) TRF2 protein complexes. (b) Quantification of the relationship between the amount of DNA compacted inside TRF2 proteins and corresponding AFM volume. The black line: the linear fit, blue lines: lower and upper bounds of the 95% prediction limit for the fit. The linear fit for this data (R2 = 0.52): V = 1766 + 2.9 Lc. The insert is a plot from averaging the volume of TRF2 complexes in each compacted DNA length window (100 bp). The error bars represent standard deviations. The red line is the fitting of the data (R2 = 0.68): V = 1284 + 3.2 Lc, where V is the volume of the TRF2 complex and Lc is the DNA length compacted inside TRF2 complexes in base pairs.
