Figure 4
DREEM imaging reveals DNA passing through small TRF2 complexes and appearing at the edge of large TRF2 complexes.
(a) The topographic AFM (left) and DREEM phase (right) images of small TRF2-DNA complexes. The volumes of these TRF2-DNA complexes are less than 500 nm3. (b) The topographic AFM (left) and DREEM phase (right) images of large TRF2 complexes (volume > 500 nm3). The XY scale bars are at 20 nm. The contrast of topographical images was adjusted with protein complexes over-saturated (data above a height of ~1 nm are in white) in order to show DNA (~0.5 nm). The 3D surface plots and cross-section analysis of these images are shown in Supplementary Figs 8 and 9.
