Figure 4 | Scientific Reports

Figure 4

From: Transcriptional Regulation of Atp-Dependent Chromatin Remodeling Factors: Smarcal1 and Brg1 Mutually Co-Regulate Each Other

Figure 4

SMARCAL1 positively regulates brg1.

(A) The architecture of brg1 promoter with RNAPII and SMARCAL1 localization. (B) The TSS1 region amplified using primer pair 5 was cloned into pGL3 basic vector and transfected into HeLa cells. Luciferase activity was measured after 36 hours and was normalized to Renilla activity. (C) SMARCAL1, RNAPII, H3K9Ac and H3K9(Me)2 occupancy on brg1 promoter was analyzed using primer pair 2 after treating HeLa cells with 2 μM doxorubicin for 10 min. The difference was significant for all with p-values  < 0.05. (D) SMARCAL1, RNAPII, H3K9Ac and H3K9(Me)2 occupancy on brg1 promoter was analyzed using primer pair 5 after treating HeLa cells with 2 μM doxorubicin for 10 min. The difference was significant for RNAPII, SMARCAL1 and H3K9(Me)2 with p values  < 0.05. GAPDH was used as the internal control in all these experiments. (E) Model explaining the regulation of brg1 gene in the absence and presence of doxorubicin by SMARCAL1.

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