Figure 1
From: An efficient Potato virus X -based microRNA silencing in Nicotiana benthamiana
Diagram of the Potato Virus X based PVX-LIC vector for VbMS.
The PVX-LIC vector was generated by introducing the LIC cassette into a T-DNA PVX vector. Artificially designed TM sequences can be cloned into PVX-LIC via the LIC reaction and expressed under the control of the CP subgenomic promoter. LB: T-DNA left border, RB: T-DNA right border, 35S: Cauliflower mosaic virus 35S promoter, NOSt: nopaline synthase terminator, RdRP: RNA dependent RNA polymerase, 25K: PVX 25K protein, 16K: PVX 16K protein, 8K: PVX 8K protein, CP: coat protein.
