Table 1 Production of recombinant VZV membrane proteins and mAbs.
Protein name | Expression system | Expression segment (aa) | Fusion tag | Expression form | Purify method | mAbs |
---|---|---|---|---|---|---|
rgE | Baculovirus | 1–537 | His tag | Secretion expression | Ni-NTA | 70 41 |
rgB | E. coli | 23–382 | His tag | Inclusion body | Electroelution | 5 |
rgH | E. coli | 518–804 | His tag | Inclusion body | Ni-NTA | 3 |
rgI | E. coli | 21–274 | GST tag | Soluble expression | Glutathione Sepharose 4B | 2 |
rgC | E. coli | 18–407 | GST tag | Soluble expression | Glutathione Sepharose 4B | 3 |
rgK | E. coli | 22–114 | GST tag | Inclusion body | Electroelution | 2 |
rgL | E. coli | 23–160 | His tag | Inclusion body | Ni-NTA | 4 |
rgM | E. coli | 2–35 | GST tag | Soluble expression | Glutathione Sepharose 4B | 3 |
rgN | E. coli | 21–49 | His tag | Soluble expression | Ni-NTA | 2 |
VZV gps | Â | Â | Â | Â | Lentil lectin Sepharose 4B | 7 |
Cell-free virus | Â | Â | Â | Â | Â | 9 |