Table 1 Production of recombinant VZV membrane proteins and mAbs.

From: Serological Evaluation of Immunity to the Varicella-Zoster Virus Based on a Novel Competitive Enzyme-Linked Immunosorbent Assay

Protein name

Expression system

Expression segment (aa)

Fusion tag

Expression form

Purify method

mAbs

rgE

Baculovirus

1–537

His tag

Secretion expression

Ni-NTA

70 41

rgB

E. coli

23–382

His tag

Inclusion body

Electroelution

5

rgH

E. coli

518–804

His tag

Inclusion body

Ni-NTA

3

rgI

E. coli

21–274

GST tag

Soluble expression

Glutathione Sepharose 4B

2

rgC

E. coli

18–407

GST tag

Soluble expression

Glutathione Sepharose 4B

3

rgK

E. coli

22–114

GST tag

Inclusion body

Electroelution

2

rgL

E. coli

23–160

His tag

Inclusion body

Ni-NTA

4

rgM

E. coli

2–35

GST tag

Soluble expression

Glutathione Sepharose 4B

3

rgN

E. coli

21–49

His tag

Soluble expression

Ni-NTA

2

VZV gps

    

Lentil lectin Sepharose 4B

7

Cell-free virus

     

9