Figure 2 | Scientific Reports

Figure 2

From: Role of brassinosteroid signaling in modulating Tobacco mosaic virus resistance in Nicotiana benthamiana

Figure 2

Effects of BR biosynthetic and signaling genes on BR-induced TMV defense.

(a) Analysis of GFP fluorescence in the gene-silenced plants. The TMV-GFP spread assay was performed on NbDWARF-, NbBRI1-, NbNSK1-, NbBAK1-, NbBIK1-, NbBSU1- and NbBES1/BZR1-silenced and control N. benthamiana plants (TRV:00) pretreated with water, BL, Bikinin or BL + Bikinin. Photographs were taken from inoculated leaves at 5 days post inoculation (dpi). (b) Quantitative real-time PCR analysis of TMV mRNA accumulation levels in inoculated leaves collected at 5 dpi. Bars represent mean and standard deviation of values obtained from three biological replicates per genotype and time point. Significant differences (P < 0.05) are denoted by different lowercase letters.

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