Figure 4
Involvement of ROS in BR-induced TMV defense.
NBT- (a) and DAB (b)-stained mock or TMV-GFP inoculated N. benthamiana leaves pretreated with water, BL or Bikinin at 2 days post inoculation (dpi). (c) H2O2 levels in mock or TMV-GFP inoculated leaves determined at 2 dpi. (d) Quantitative real-time PCR analysis of TMV mRNA accumulation levels in NbRBOHA-, NbRBOHB-, NbRBOHA&RBOHB-silenced and control plants (TRV:00) pretreated with water or BL at 5 dpi. (e) TMV-GFP spread in gene-silenced leaves pretreated with water or BL was photographed at 5 dpi. “Mock” means seedlings not infected with TMV-GFP. Bars represent mean and standard deviation of values obtained from three biological replicates per genotype and time point. Significant differences (P < 0.05) are denoted by different lowercase letters.
