Figure 7
Transient expression of SIPK enhances BR-induced oxidative burst and virus resistance.
H2O2 levels (a), expression of NbRBOHB (b) and activities of the antioxidant enzymes SOD (c) and APX (d) in NbSIPK-, NbRBOHB-silenced and control (TRV:00) N. benthamiana plants (pretreated with BL and inoculated with TMV-GFP) which were infiltrated with 35S: NbSIPK or 35S:00. (e) TMV-GFP spread was performed in treated plants as described in (a–d). Photographs were taken from inoculated leaves at 5 days post inoculation (dpi). (f) Quantitative real-time PCR analysis of TMV mRNA accumulation levels in inoculated leaves collected at 5 dpi. Bars represent mean and standard deviation of values obtained from three biological replicates per genotype and time point. Significant differences (P < 0.05) are denoted by different lowercase letters.
