Figure 7
ROS-dependent ER stress is involved in the miltirone-mediated ROS production and apoptosis.
(a) Cells were treated with 7.5 μM miltirone for 12 h in the presence or absence of 5 mM NAC and the expression of GRP78, caspase-12 and β-actin was analyzed; n = 3. The bands were excised from different gels which were run under the same electrophoresis condition. (b–e) Cells were treated with 7.5 μM miltirone for 12 h in the presence or absence of 100 μM TUDCA and the cell viability (b), apoptosis (c), ROS (d) and MMP (e) were detected. Data were presented as Mean ± SD. The differences were significant at *p< 0.05, **p< 0.01 miltirone vs. co-treatment with miltirone and TUDCA.
