Figure 2
NQC is more cytotoxic in cervical cancer cells in comparison to normal epithelial cells.
Cells were treated with different concentrations of QC and NQC and assays were carried out according to materials and methods described in the text. (a) MTT cell viability assay in HeLa and Vero cells. (b) Clonogenic cell survival assay in HeLa cells. (c) Tabular representation of IC50 values of QC and NQC in different cells. (d) Detection of apoptosis by DAPI nuclear staining. Images (scale bar 10 μm) were taken with fluorescence microscope with 40× magnification (Nikon, Japan). Graphical representation of apoptotic and non-apoptotic DAPI stained nuclei in NQC (e) and QC (f) treated HeLa cells. (g) Regulation of cell cycle profile in HeLa cells. (h) Representative expressions of pro- and anti-apoptotic markers in HeLa whole-cell lysate. GAPDH severed as loading control. Data from quantification immunoreactive signal is represented as a fold to control and statistical significance was determined by paired t test, (*p < 0.05), (**p < 0.005), (***p < 0.001).
