Figure 4
Inhibition of HH-GLI cascade by NQC in HeLa cells is through GLI1.
Cells were treated with different concentrations of NQC for 48 h and then assays were carried out according to materials and methods described in text. (a) Expressions of representative proteins of WNT-TCF, HH-GLI and NOTCH signaling cascade in the whole cell extracts. GAPDH serves as loading control. (b) GLI1 promoter activity. Luciferase activity was measured after transiently transfecting PGL2-GLI-luc promoter plasmid followed by NQC treatment (c) GLI-mRNA expressions measured by quantitative RT PCR. GAPDH serves as loading control. (d) Expressions of proteins of HH-GLI signaling after NQC treatment in HeLa GLI1 knock down cells. α - tubulin serves as loading control. (e) GLI1 promoter activity in HeLa GLI1 knock down cells. GLI1 knock down cells were transiently transfected with PGL2-GLI1-luc promoter plasmid and then luciferase activity was measured after NQC exposure. (f) Regulation of cell cycle profile in NQC treated HeLa GLI1 knock down cells. Data is the representation of three independent experiments. Statistical significance was determined by paired t test (*p < 0.05), (**p < 0.005), (***p < 0.001).
