Figure 6

NQC caused cytotoxicity in cervical CSCs.
Cells were exposed to different concentrations of NQC for 48 h and then different assays were carried out. (a) Comparative cell survival of PEMT and HeLa cells. (b) Change in expression of HH-GLI components at protein level in PEMT cells. GAPDH was used as the loading control. (c) Cell cycle profile of NQC exposed PEMT cells. (d,e) GLI1 reporter activity in PEMT and GLI1-knock down PEMT cells after NQC treatment, respectively. Data presented is the mean ± SD of three independent experiments. Statistical significance was determined by paired t test (*p < 0.05), (**p <0.005).