Figure 2: Brife phenotypic identification of triple gene-modified piglets.
From: One-step generation of triple gene-targeted pigs using CRISPR/Cas9 system
(A) DJ-1 immunofluorescence analysis of the fibroblasts of WT and two gene modified piglets. The fibroblasts of gene-modified piglets are negative for anti-DJ-1 staining; in contrast, the WT controls show positive staining in the fibroblasts. (B) Western blot analysis showed the absence of DJ-1 protein expression in the fibroblasts of these two gene modified piglets. (C) Quantitation of parkin and PINK1 gene expression in the fibroblasts of WT and two gene modified piglets. parkin and PINK1 gene expression was dramatically decreased in the gene modified-derived pig fibroblast cells compared with wild-type cells based on real-time RT-PCR. The graph demonstrates the results of the gene expression levels normalized against loading controls (arbitrary units, wild type = 1). (D) Comparison of oxidative stress defense gene expression in wild type and gene modified-derived pig fibroblast cells by real-time RT-PCR. The graph demonstrates the results of the gene expression levels normalized against loading controls (arbitrary units, wild type = 1). *p < 0.05.
