Figure 1
From: Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism
In vivo effects of alp14 deletion versus alp7 deletion (A) Spinning disc microscopy of GFP-labelled MTs in control, alp7 and alp14 deletion strains of S. pombe. (B,C) Deletion of alp7 or alp14 significantly reduces the number of interphase microtubule arrays (IMAs) in each cell, at both 26.5 °C and 35 °C (Kruskal-Wallis p < 0.0001, Dunn’s post test p < 0.001)). See Movie S1. (D) MT growth is inhibited by alp7 or alp14 deletion. Mean MT growth rates are significantly different at both 26.5 °C (ANOVA, p < 0.0001) and 35 °C (p < 0.0001). Wild type is significantly faster than either alp7 or alp14 deletions at both 26.5 °C (p < 0.001) and 35 °C (p < 0.001). alp7 MT growth rate in alp7 deletions is not significantly different from that of alp14 deletions at 26.5 °C (p < 0.001) or 35 °C (Tukey post test, p < 0.001). (E) MT shrinkage is inhibited by alp7 or alp14 deletion. Shrinkage rates are significantly different at 26.5 °C (ANOVA, p < 0.0001) and 35 °C (p < 0.0001). Wild type is significantly faster than either alp7 or alp14 deletions at both 26.5 °C (p < 0.001) and 35 °C (Tukey post test, p < 0.001). alp7 deletion is not significantly different from alp14 deletion at 26.5 °C (p < 0.001) and 35 °C (p < 0.001). (F) Catastrophe and rescue are not significantly different in any of the genotypes, at either 26.5 °C or 35 °C (26.5 °C shown; ANOVA, respectively, p = 0.0784 and p=0.3615). (G) alp14 deletion but not alp7 deletion increases pausing. Growth times are significantly different (ANOVA, p = 0.0102). MTs in wild type cells spend significantly longer in growth than those in alp14 deletions at p < 0.05. Differences are not significant (p < 0.05) between wild type and alp7 deletions or between alp14 and alp7 deletions (Tukey post test). Times spent in shrinkage are significantly different (ANOVA, p = 0.0006). MTs in wild type and alp7 deletions spend significantly longer in shrinkage than those in alp14 deletions (p < 0.01). Differences between wild type and alp7 deletions are not significant at (Tukey post test, p < 0.01). Percentage times spent paused are significantly different (ANOVA, p = 0.0022). The percentage of total time MTs spend paused is significantly longer in alp14 deletions than in wild type or alp7 deletions (p < 0.05). Differences between wild type and alp7 deletions are not significant (Tukey post test, p < 0.05). Error bars are SEM.
