Table 2 Microtubule growth rate, mean length & catastrophe frequency in Alp14 and Alp7 Alp7 greatly enhances the action of Alp14, producing a further increase in growth rate and a substantial increase in MT length together with reduced catastrophe frequency at MT plus ends.

From: Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism

 

Plus end growth nm s−1

Plus end catastrophe frequency min−1

MT length μm

Minus end growth nm s−1

Minus end catastrophe frequency min−1

50 nM Alp14

10.3 ± 3.2 (39)

0.16

3.6 ± 4.4 (24)

2.6 ± 1.6 (27)

0.15

80 nM Alp14

13.4 ± 4.0 (38)

0.12

6.5 ± 5.7 (29)

2.3 ± 1.0 (19)

0.1

50 nM Alp14 + 100 nM Alp7

15.2 ± 4.2 (9)

0.04

18.6 ± 12.4 (8)

2.5 ± 1.3 (11)

0.15

  1. Mean values ± SD (n). Mean growth rates at plus ends are significantly different (ANOVA, p = 0.0001). The growth rate of either 80 nM Alp14 alone or 50 nM Alp14 + 100 nM Alp7 were both significantly faster than 50 nM Alp14 alone. There was no significant difference between the growth rate of 80 nM Alp14 alone or 50 nM Alp14 + 100 nM Alp7 (Tukey post test, p < 0.01). There was no significant difference between the mean growth rates at MT minus ends (ANOVA, p = 0.7656). The mean MT lengths were significantly different (ANOVA, p < 0.0001). 50 nM Alp14 + 100 nM Alp7 was significantly longer than either 80 nM or 50 nM Alp14 alone. There was no significant difference between 50 and 80 nM Alp14 (Tukey post test p < 0.001).
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