Figure 3: Western blot analysis of recombinant MsDorsal-RHD, MsRel2-RHD, MsRel2A and MsRel2B in S2 and Sf9 cells. | Scientific Reports

Figure 3: Western blot analysis of recombinant MsDorsal-RHD, MsRel2-RHD, MsRel2A and MsRel2B in S2 and Sf9 cells.

From: Co-expression of Dorsal and Rel2 Negatively Regulates Antimicrobial Peptide Expression in the Tobacco Hornworm Manduca sexta

Figure 3

V5-tagged MsDorsal-RHD, Flag-tagged MsRel2-RHD, V5-tagged MsRel2A and MsRel2B (full-length proteins) were transiently expressed in S2 or Sf9 cells, and cytoplasmic (Cp) and nuclear (Nu) proteins were prepared separately for Western blot analysis using monoclonal anti-V5 or anti-Flag antibody. Panels A and B: DI-RHD-V5 and Rel2-RHD-Flag, respectively; Panel C: MsRel2A-V5 and MsRel2B-V5 from S2 cells.

Back to article page