Figure 8: MsDorsal-RHD and MsRel2-RHD together negatively regulate M. sexta AMP gene promoters.

MsDorsal-RHD (Dl-RHD) and MsRel2-RHD (Rel2-RHD) were expressed separately or together in S2 (A) or Sf9 (B) cells. Activation of D. melanogaster (A) or M. sexta (B) AMP gene promoters were determined by Dual-Luciferase® Reporter Assay System. Drs, drosomycin; Def, defensin; Cec, cecropin; Att, attacin; Dpt, diptericin; Dro, drosocin; Met, metchnikowin; Leb, B. mori lebocin-4. Bars represent the mean of three independent measurements ±SEM.