Figure 2: NOTCH1 pathway and EMT markers in HT29 and HCT116 cells under inflammatory conditions. | Scientific Reports

Figure 2: NOTCH1 pathway and EMT markers in HT29 and HCT116 cells under inflammatory conditions.

From: Inflammation increases NOTCH1 activity via MMP9 and is counteracted by Eicosapentaenoic Acid-free fatty acid in colon cancer cells

Figure 2

(a) Western Blot analyses for NICD and JAG1 in HT29 and HCT116 exposed to CM for 12 h. β-ACTIN was used as housekeeping protein. Each experiment was repeated at least three times. (b) qRT-PCR for JAG1 and NRARP, HES1 and ATOH1 in HT29 and HCT116 cells incubated in CM; Statistical significance was calculated on logarithmic transformed values using unpaired t-test, (n = 3, N = 4). *p < 0.05; **p < 0.01; ***p < 0.001. GAPDH was used as housekeeping gene. (c) Western Blot analyses for ZEB1, MMP9, and CDH1 in HT29 and HCT116 exposed to CM for 12 h. β-ACTIN was used as housekeeping protein. Each experiment was repeated at least three times. (d) qRT-PCR for ZEB1, MMP9 and CDH1 in HT29 and HCT116 cells incubated in CM. GAPDH was used as housekeeping gene. Statistical significance was calculated on logarithmic transformed values using unpaired t-test, (n = 3, N = 4 for HT29 and n = 3, N = 3 for HCT116). *p < 0.05; **p < 0.01; ***p < 0.001.

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