Figure 1
Effect of oligofructose supplementation on maternal body weight, energy intake, body composition, glycemia and insulinemia.
During gestation and lactation, diet-induced obese dams were fed either a high-fat/sucrose diet (HFS obese control group), the high-fat/sucrose diet supplemented with 10% wt/wt oligofructose (OFS group), or a restricted amount of the high-fat/sucrose diet in order to match body weight to the OFS dams (WM). A lean reference group was maintained on control AIN-93 G diet through pregnancy and lactation for body weight and body composition measurements but was not included in statistical analysis. Maternal glycemia and insulinemia was determined from blood collected during oral glucose tolerance tests (OGTTs) performed on gestation day 14 and lactation day 19. Dams were fasted overnight (12 h) and an OGTT was performed after gavage with glucose (2 g/kg body weight). Blood samples were collected at 0, 15, 30, 60, 90 and 120 minutes during the OGTT. (a) Maternal body weight during gestation and lactation (HFS, n = 11; OFS, n = 12; WM, n = 9). (b) Maternal energy intake during gestation and lactation (HFS, n = 7; OFS, n = 12; WM, n = 9; Independent-Samples Kruskall-Wallis Test with adjusted significance where *P < 0.05 compared to all other groups). (c) Maternal fat mass and percent body fat at weaning (HFS, n = 10; OFS, n = 9; WM, n = 8). (d,e) Maternal glycemia and insulinemia (HFS, n = 7; OFS, n = 12; WM, n = 9). (f) Area under the curve (AUC) for glucose and insulin concentrations during the OGTTs (HFS, n = 7; OFS, n = 12; WM, n = 9; Student’s t-test). Graphs represent mean +/− SEM. Mean values without a common letter are significantly different using one-way ANOVA (P < 0.05). For body weight, glycemia and insulinemia measurements, the data were analyzed using repeated measures one-way ANOVA, where the between subjects factor was maternal diet and the within-subjects factor was time. If a statistically significant interaction was observed, a one-way ANOVA between groups was performed.
