Figure 4

Oligofructose feeding increases circulating levels of peptide YY (PYY), glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2).

Blood samples for PYY and GLP-1 area under the curve were collected during the glucose tolerance tests performed pre-pregnancy, on gestation day 14 and lactation day 19 from diet-induced obese dams fed either a high-fat/sucrose diet (HFS obese control group), the high-fat/sucrose diet supplemented with 10% wt/wt oligofructose (OFS group), or a restricted amount of the high-fat/sucrose diet in order to match body weight to the OFS dams (WM) during pregnancy and lactation. Portal blood samples for GLP-1 and GLP-2 measurement were collected at euthanasia of the dams. (a,b) Relative increase of PYY and GLP-1 area under the curve (AUC) on gestation day 14 and lactation day 19 compared to pre-pregnancy AUC (PYY: HFS, n = 11; OFS, n = 12; WM, n = 9; GLP-1: HFS, n = 11; OFS, n = 10; WM, n = 9). (c,d) Portal plasma GLP-1 and GLP-2 concentration (GLP-1: HFS, n = 10; OFS, n = 9; WM, n = 7, GLP-2: HFS, n = 10; OFS, n = 9; WM, n = 8). Means without a common letter are significantly different using one-way ANOVA (P < 0.05). For relative AUC measurements, the data were analyzed using repeated measures one-way ANOVA, where the between subjects factor was maternal diet and the within-subjects factor was timepoint (pre-pregnancy, gestation and lactation). If a statistically significant interaction was observed, one-way ANOVAs between groups was performed. *P < 0.05 Independent-Samples Kruskall-Wallis Test with adjusted significance.