Figure 6
Metabolomic analysis of maternal serum collected during lactation.
Comparison of maternal serum metabolites from dams fed a high-fat/sucrose diet (HFS obese control group), the high-fat/sucrose diet supplemented with 10% oligofructose (OFS group), or a restricted amount of the high-fat/sucrose diet to weight-match to the OFS group (WM) during gestation and lactation. (a–c) Pairwise OPLS-DA loadings plots comparing maternal serum metabolites from samples collected on lactation day 19. Metabolites included in the OPLS-DA modeling were selected following preliminary pairwise univariate analysis of the 57 metabolites identified and quantified by 1H NMR spectroscopy. Bold metabolites represent the variables that contributed most to the discrimination of dietary groups, which were identified using a combination of VIP > 1 and p(corr) > 0.4, with those contributing most to the separation located furthest from the center. The directions of the arrows indicate the maternal group corresponding to increased levels of those metabolites. HFS, n = 10; OFS, n = 9; WM, n = 8.
