Figure 2: Trajectories of LFA-1 nanoclusters are visually affected by SMase. | Scientific Reports

Figure 2: Trajectories of LFA-1 nanoclusters are visually affected by SMase.

From: Changes in membrane sphingolipid composition modulate dynamics and adhesion of integrin nanoclusters

Figure 2

(A) Widefield image of a living THP-1 cell (left) and selected frame from a movie recorded at 100ms/frame in TIRF mode (middle). Bright spots correspond to individual LFA-1 nanoclusters labeled with TS2/4-Atto647N. Examples of different trajectories as they are tracked on the cell membrane of THP-1 cells over 200 frames (right). Scale bar: 10 μm. (B) Representative trajectories of LFA-1 nanoclusters in unperturbed (left) and SMase (right) treated cells, illustrating different lateral mobilities.

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