Figure 4

Protein identification and protein quantitation summary from TMT labelling of trophozoites co-incubated with the IEC monolayer (CI IEC) and with host soluble factors alone (HSF).

(A) Outline of protein identification, differentially expressed proteins and protein quantitation FDR for the dataset. (B) Volcano plots illustrating the dual criteria for differentially expressed proteins. The x-axis represents log fold change with the vertical blue lines indicating 1.2 and 0.8 ratio, while the -log p value is plotted on the y-axis with proteins above the red horizontal line indicating significance ≤0.05. Each data point represents a single identified protein. Proteins within the upper and outer quadrants meet both the fold change and p-value cut-off and are therefore considered as differentially expressed. (C) Proportional venn diagrams showing overlap between up-regulated and down-regulated proteins in trophozoites between CI IEC and HSF treatments.