Figure 2: Plumericin arrests VSMC in G0/G1 of the cell cycle. | Scientific Reports

Figure 2: Plumericin arrests VSMC in G0/G1 of the cell cycle.

From: Plumericin inhibits proliferation of vascular smooth muscle cells by blocking STAT3 signaling via S-glutathionylation

Figure 2

(a) Serum-deprived quiescent (quiesc.) VSMC were pretreated with solvent (DMSO) or plumericin as indicated for 30 min and then exposed to serum (10% NBS) for 16 h before cell nuclei were stained with PI and analyzed by flow cytometry. Compiled evaluation of three independent experiments is shown (mean +SD; *p < 0.05 (compared groups (% cells in G1 phase) are indicated by lines), ANOVA, Bonferroni). (b) Quiescent VSMC were pretreated with 3 μM plumericin (Plum) for 30 min, exposed to serum (10% NBS) for the indicated periods of time and then lysed. Total cell lysates were subjected to western blot analysis for phospho Rb (Ser 807/811) and actin as loading control. Representative cropped blots (for raw data see Supplemental Fig. 9) and compiled densitometric data from three independent experiments are depicted (n = 3, *p < 0.05 (compared groups are indicated by lines), ANOVA, Dunnett).

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