Figure 3: Plumericin and Stattic inhibit serum-induced cyclin D1 upregulation in VSMC. | Scientific Reports

Figure 3: Plumericin and Stattic inhibit serum-induced cyclin D1 upregulation in VSMC.

From: Plumericin inhibits proliferation of vascular smooth muscle cells by blocking STAT3 signaling via S-glutathionylation

Figure 3

(a) Quiescent VSMC were pretreated with 3 μM plumericin (Plum) for 30 min and then exposed to serum (10% NBS) as indicated. After lysis, total cell lysates were subjected to immunoblot analysis for cyclin D1 and actin. Representative cropped blots (for raw data see Supplemental Fig. 9) as well as compiled densitometric data from three independent experiments are depicted (n = 3, *p < 0.05 (compared groups are indicated by lines), ANOVA Bonferroni). Quiescent VSMC were pretreated with plumericin (3 μM, Plum) (b) or Stattic (10 μM) (c) for 30 min prior to exposure to serum (10% NBS) for 6 h. RNA was extracted, transcribed to cDNA, subjected to qPCR analysis for cyclin D1 as target gene or HRPT-1 and 18S rRNA as reference genes. The graphs depict compiled data (2−ΔΔCt) from three independent experiments each. (*p < 0.05; (compared groups are indicated by lines), ANOVA, Bonferroni).

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