Figure 6: Exogenous addition of GSH, in form of cell permeable ethyl ester (GEE), hinders plumericin to inhibit Stat3 phosphorylation, cyclin D1 upregulation and proliferation in VSMC. | Scientific Reports

Figure 6: Exogenous addition of GSH, in form of cell permeable ethyl ester (GEE), hinders plumericin to inhibit Stat3 phosphorylation, cyclin D1 upregulation and proliferation in VSMC.

From: Plumericin inhibits proliferation of vascular smooth muscle cells by blocking STAT3 signaling via S-glutathionylation

Figure 6

Quiescent VSMC were treated with 2 mM GEE for 18 h and thoroughly washed. Cells were then treated with plumericin (Plum, 3 μM) for 30 min and stimulated with serum (10% NBS) for 10 min, 8 h or 48 h before subjected to western blot analysis for (a) phospho Stat3 (Tyr705), Stat3, and actin, or (b) cyclin D1 and tubulin or to (c) biomass stain by crystal violet. Depicted blots are cropped (for raw data see Supplemental Fig. 9) and representative for three independent experiments. Bar graphs depict compiled data from the three independent experiments (*p < 0.05 (compared groups are indicated by lines), ANOVA, Bonferroni).

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