Figure 4

The intact C-terminus rather than a small motif is essential for collembolan Pax6 function.

(A,B) Diagram of constructs with gradual CT deletions (numbers indicate the amino acid positions of Fc-Pax6 (red) and Cd-Pax6 (blue)), corresponding to different strengths of transactivation indicated by “★” (“⋆” denotes half strength of the “★”). (C–L’) Phenotypes of transgenic Drosophila induced by deletion constructs with different CT lengths of Fc-Pax6 and Cd-Pax6 driven by dpp-GAL4 (C–L) and ap-GAL4 (C’–L’). Arrows indicate ectopic eyes. (M) Comparison of the positions of ectopic eyes on transgenic Drosophila induced by deletion constructs with different CT lengths. The construct names connected by “|” indicate the same alignment positions. “ND” indicates that there is no corresponding construct. For the position, “+” indicates that ectopic eyes exist, “−” indicates a lack of ectopic eyes and “ND” implies no data. (N) Comparison of the sizes of ectopic eyes.