Figure 5

Different transactivation ability between Cd-Pax6 and Fc-Pax6 is determined by their respective C-terminus.

(A) Schematic summaries of the original Fc-Pax6 (red) and Cd-Pax6 (blue), along with chimeric constructs with whole or partial CT substitutions, corresponding to different strengths of transactivation indicated by “★” (“⋆” denotes half strength of the “★”). (B–E’) Phenotypes of transgenic Drosophila induced by chimeric constructs with CT region substitutions of Fc-Pax6 and Cd-Pax6 driven by dpp-GAL4 (B–E) and ap-GAL4 (B’–E’). Arrows indicate positions of ectopic eyes. (F) Comparison of the position of ectopic eyes induced by chimeric constructs with CT region substitutions (“+” indicates that ectopic eyes exist and “−” indicates a lack of ectopic eyes). (G) Comparison of the sizes of ectopic eyes.