Figure 6
From: ITF2357 transactivates Id3 and regulate TGFβ/BMP7 signaling pathways to attenuate corneal fibrosis
ITF2357 shows inhibitory effect on TGFβ/Smad signaling pathway.
To elucidate mechanistic effects of ITF2357, key members of TGFβ/SMAD pathway were investigated. ITF2357 attenuated the TGFβ-induced expression of (A) TGFβRII protein and (D) Smad4 mRNA and (E) protein. (F) Smad7 mRNA was increased in TGFβ + ITF2357 group, but was reduced in (G) protein expression. Phosphorylation of (B) Smad2 and (C) Smad3 were both downregulated in the presence of ITF2357. (H,I) P4HA1 expression was increased with TGFβ treatment, which was significantly reduced with addition of 250 nM ITF2357. *p < 0.05; **p < 0.01 and ***p < 0.001 against control; #p < 0.05; ##p < 0.01 and ###p < 0.001 against TGFβ. There were n = 6 samples for mRNA expression and n = 4 samples for protein expression in each group. Error bars represent SEM.
