Figure 7
From: ITF2357 transactivates Id3 and regulate TGFβ/BMP7 signaling pathways to attenuate corneal fibrosis
ITF2357 increases BMP7 production in BMP7/Smad signaling pathway.
(A) BMP7 transcript was upregulated by ITF2357, but attenuated with TGFβ. (B) BMP7 protein was undetectable in the control and TGFβ as seen with ELISA assay, but showed a ~15-fold increase in the presence of ITF2357 and ~40-fold increase in the ITF2357 + TGFβ group. TGFβ stimulation to pHCSFs decreased (C) Smad1, (D) Smad5 and (E) Smad8 expression, which was restored by the treatment of ITF2357. However, (F) pSmad1/5/8 protein only showed a slight increase in the TGFβ + ITF2357 group. Moreover, we found no significant changes in (G) Smad6 protein in any of the group studied. *p < 0.05; **p < 0.01 and ***p < 0.001 against control; #p < 0.05 and ###p < 0.001 against TGFβ. There were n = 6 samples for mRNA expression and ELISA assay whereas n = 4 for protein expression. Error bars represent SEM.
