Figure 6 | Scientific Reports

Figure 6

From: The binding capability of plasma phospholipid transfer protein, but not HDL pool size, is critical to repress LPS induced inflammation

Figure 6

The role of macrophage derived PLTP on LPS induced TNFα expression and TLR4 activation.

(A) BMDM with or without LPS stimulation were stained with 1 μg/mL TLR4-MD-2 complex antibody for 1 hour on ice, then washed with ice cold PBS for 3 times and then analyzed on Flow Cytometer. (B) Gated cell counting and analysis of positive signal. (C) 200 ng/ml LPS was incubated for 60 min with concentrated cultured media (CCM) from WT or PLTP−/− BMDM and then the mixture were added into PLTP−/− BMDM for another 24 h culture. The medium was harvested for TNFα level determination by ELISA. (D) the CCM from WT or PLTP−/− BMDM was harvested for PLTP activity determination. Data are expressed as the mean ± SD (n = 4). *P < 0.05; NS, no significance.

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