Table 1 Calibration equations, correlation coefficients (r2), linear ranges, limit of detection (LOD), limit of quantitation (LOQ) and recovery of IS.
From: Quantitative profiling of sphingolipids in wild Cordyceps and its mycelia by using UHPLC-MS
IS | Calibration equations | r 2 | Linear range (nM) | LOD (nM) | LOQ (nM) | Low# (n = 6) | Medium& (n = 6) | High* (n = 6) | Mean Recovery (%) | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Recovery (%) | RSD (%) | Recovery (%) | RSD (%) | Recovery (%) | RSD (%) | |||||||
IS-1 | Y = 443.61X – 1920.10 | 0.9992 | 3.34–3340.00 | 0.025 | 0.835 | 96.72 | 3.50 | 83.13 | 1.83 | 81.31 | 2.37 | 87.05 |
IS-2 | Y = 86.64X – 960.46 | 0.9977 | 8.35–3340.00 | 1.670 | 6.680 | 90.23 | 3.56 | 89.33 | 1.28 | 71.59 | 1.61 | 83.72 |
IS-3 | Y = 92.01X – 1303.30 | 0.9977 | 16.70–3340.00 | 3.340 | 16.700 | 88.98 | 4.81 | 75.43 | 2.30 | 80.69 | 1.27 | 81.70 |
IS-4 | Y = 413.61X – 12809.00 | 0.9988 | 8.35–3340.00 | 0.025 | 1.670 | 104.12 | 3.07 | 101.40 | 4.46 | 94.94 | 1.39 | 100.15 |
IS-5 | Y = 1171.30X + 12114.00 | 0.9994 | 3.34–3340.00 | 0.005 | 0.017 | 100.65 | 2.99 | 97.01 | 2.10 | 100.60 | 1.62 | 99.42 |
IS-6 | Y = 74.38X – 2785.00 | 0.9983 | 8.35–3340.00 | 1.670 | 6.680 | 73.06 | 3.24 | 94.43 | 2.28 | 97.81 | 4.84 | 88.43 |
