Figure 1

Comparision of primary and secondary structures of Bc3, Bc4 and Bc5 RNA.
(a) Multiple sequence alignments of Bc3, Bc4 and Bc5 compiled by BioEdit. Aptamers and expression platforms were underlined with solid and dash lines, respectively. (b) Secondary structure comparisons of Bc3, Bc4 and Bc5 aptamers with Vc2 aptamer. Conserved motifs such as tetraloop (blue motif in stem P2), tetraloop receptor (green motif in stem P3) and G·C base pair (C base in stem P2 and G base in stem P3 were drawn in magenta) connecting P2 with P3 were all colored to facilitate comparison. c-di-GMP was drawn in cyan and its interacting bases drawn in red.