Figure 2 | Scientific Reports

Figure 2

From: Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter

Figure 2

Characterization of tandem c-di-GMP-I in E. coli Trans5α using lacZ as a reporter.

(a) Effect of different riboswitches on RSA values. Strains were cultured at 28 °C and β-galactosidase assays were carried out according to the procedure described in the Methods section. RSAs were calculated using formula listed in the Methods section. Data were subjected to one-way analysis of variance (ANOVA) using Bonferroni test by the OriginLab 8.0 software. *P value < 0.05; ***P value < 0.001; ****P value < 0.0001. All Data are averages of three independent experiments (error bars are standard deviation from mean values). (b) Blue-white spots using the same-batch strains for β-galactosidase assay. These colonies were grown at 28 °C following the procedure in the Methods section. Strain information were listed in Table 1.

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